The study aimed to evaluate the superovulatory response and conception rate by applying the technique of non-surgical embryo transfer in dairy cows of a commercial farm in the Lima milkshed. Eight donor cows were used and distributed in two working years. Donors were synchronized with estrogens, progestagens and luteolitic agents, superovulated with 400 mg of FSH, and inseminated with two doses of frozen semen. The recipient animals (n=28) were also distributed in two years. The Pre-synch protocol was used for estrus synchronization in the first year and the CIDR-synch protocol in the second year. The expected day of heat presentation coincided for both donors and recipients. The recovery of embryos was done seven days post service where embryos were classified and immediately transferred. Pregnancy diagnosis by transrectal ultrasonography was done at day 28 post transfer. A total of 23 embryos were obtained. The embryo recovery rate was 36.5% with high variation between years. Seventeen embryos were transferred and the conception rate was 40% with high variation between years (22.2 and 66.7%). The varied individual response of the donors affected the embryo recovery rate and the synchronization protocol affected conception rate.