Themain purpose of this study is to investigate the in vitro toxic effects of 5 Bothrops spp. snake venoms, which are part of the antigenicmixture used for the production of Brazilian antivenom, and evaluate their correlation with the in vivo toxic activities of Bothrops spp. venoms. The correlation analysis could be helpful for the replacement of living animals experimentation for in vitro bioassay. Cytotoxicity, L-amino acid oxidase (LAAO), proteolitic (serine andmetalloproteinase), hyaluronidase (Hyal), and phospholipase A2 (PLA2) activities were estimated and the correlation coefficient was determined for each activity in relation to lethality, edema, hemorrhage and necrosis induced in live animals by B. jararaca, B. alternatus, B. jararacussu, B. neuwiedi, and B. moojeni venoms. The lethal activity inmice was highly related to Hyal activity (r=0.94, p<.05), edema related to PLA2 activity (r=0.94, p<.05), whereas the necrotizing activity showed high correlation with LAAO activity (r=0.83, p<.05). A very significant correlation between in vitro cytotoxicity and LAAO activities was also observed (r=0.97, p<.05).