The effect of shoot tip pre-culture on media with varying sucrose levels (0.0-0.6 M) followed by treatment with Plant Vitrification Solution 2 [PVS2] (15, 30, 45, 60 min) was evaluated with six sweet potato accessions [Ipomoea batatas (L.) Lam.], using the "PVS2-droplet vitrification" cryopreservation method. No significant differences in the percent of shoot regeneration were observed when shoot tips were pre-cultured on sucrose concentrations ranging from 0.1 to 0.4 M. The highest mean shoot regeneration rate of 57.7 ± 5.5% after rewarming from cryopreservation (+LN) was obtained with a sucrose concentration of 0.35 M. Intermediate exposure times in PVS2 of 30 and 45 min showed statistically better results than shorter or longer exposure, with a maximum mean shoot regeneration (+LN) of 68.5 ± 4.5% with a 30 min exposure. Screening a separate larger group of 24 sweet potato accessions with a 0.35 M sucrose pre-culture and 30 min PVS2 treatment (+LN) resulted in shoot formation rates ranging from 1.7 to 66%.