The aim of the study was to determine the frequency and characterize testicular anatomo-histopathological alterations in alpacas slaughtered in Nuñoa, Puno. Macroscopic examination of the testes was carried out in 177 alpacas, which 102 showed smaller size and low weight, and also uni or bilateral epididymal cysts. Tissue samples were fixed with the Bouin solution during 28 hours and stained with Haematoxylin and Eosin, Periodic Acid Schiff and Masson's Trichromic stain. The frequency of testicular alterations was 13.7%, corresponding to nonspecific lymphocytic infiltration (7.8%), testicular absence (2.9%), testicular degeneration (1.0%), hidrocele (1.0%), and teratoma (1.0%). Besides, there was 32.2% of epididymal cysts, but without statistical association with age or breed.