This paper aims to contribute to a better understanding of the Knowledge-based Economy spatial distribution across the European Union (EU) regions (NUTS II), linking recent research approaches on innovation and structural change with approaches to regional economics. As a means of conducting this research, we classify economic activities according to six sectors based on their knowledge/technology intensity. Our results show that the higher the knowledge/technology content of the economic activity, the higher the concentration level of the activity. We find that some service activities (those considered knowledge intensive) have similar concentration levels to those operating in high or medium tech manufacturing. With regard to specialization, the most outstanding result is the strong presence of high and medium knowledge intensive service activities in metropolitan/capital regions. In general, our results reinforce the notion that an oligocentric model persists in Europe, with the southern German regions leading high and medium tech manufacturing, the south-east of England leading in high knowledge-intensive services, and with the mid-core model exemplified by the European metropolitan archipelago (particularly capital cities) in both northern and southern Europe.