Minidosimeters of 2-methyalanine (2MA) with millimeter dimensions were produced and tested for small radiation field dosimetry. Their performance was assessed by measuring the relative output factor (ROF), beam profile (BP) and penumbra width values and were determined for square fields of 0.5 × 0.5, 1 × 1, 3 × 3, 5 × 5 and 10 × 10 cm2. These results were compared with those obtained for Kodak X-Omat V radiographic film. The 2MA minidosimeters (mini2MA) were irradiated with 6 MV X-rays Varian/Clinac 2100 linear accelerator with SSD of 100 cm and depth of 1.5 cm (depth for build-up equilibrium). EPR measurements were made with a K-Band (24 GHz) spectrometer. The ROF and BP results demonstrate that the dimensions of the mini2MA are adequate for the field sizes used in this experiment. The results for penumbra width indicate that the spatial resolution of the mini2MA is comparable with that of radiographic film. © 2007 Elsevier Ltd. All rights reserved.