Monitor of natural radionuclides in aquatic raw is very important to guaranty the normal control level in the environmental medium and the human. In this report were available the concentration of 226Ra and 228Ra in Cananeia's underground water, Iguape and Comprida Island into the south region of Sao Paulo state, by gross alpha and beta counting method. The concentration of 226Ra was variety into the interval 0.80 to 23 mBqL -1 in April/2009 (rain period) and into the interval 0.63 to 12 mBqL -1 in August/2009 (dried period). The concentration of 228Ra was variety into the interval 18 to 39 mBqL-1 in April/2009 and into the interval 15 to 72 mBqL -1 in August/2009. The Activities ratio of 228Ra/ 226Ra was variety into the interval 3.3 to 31.7 at rain period and 1.1 to 26.7 at dried period. To compare the yield values of 226Ra and 228Ra these show high activity of the 228Ra isotope. The calculate dose no arising the reference of effective dose level available by WHO.