Title
Seroprevalence neospora caninum in bovines of Lima and comparison between ELISA and IFAT
Other title
Seroprevalencia de Neospora caninum en bovinos de Lima y comparación entre ELISA e IFI
Date Issued
01 January 2018
Access level
open access
Resource Type
journal article
Publisher(s)
Universidad Nacional Mayor de San Marcos
Abstract
The objective of this study was to evaluate the prevalence of antibodies against Neospora caninum in dairy cattle farms of the department of Lima, Peru, and the conconrdance between two diagnostic techniques used for the detection of antibodies in serum: enzyme-linked immunosorbent assay (ELISA) and immunofluorescence antibody test (IFAT). The study was conducted between June 2015 and December 2016 in serum samples of 3407 dairy cattle from 101 farms in Lima (Barranca, Huaura, Huaral, Canta, Lima, Huarochirí, Cañete and Yauyos). The prevalence of infection to N. caninum was 31.0% (1023/3407) by ELISA and 29.9% (1018/3407) by IFAT. The concordance between both diagnostic techniques was good (K=0.98) and statistically significant (p<0.001). Likewise, 69 of the 101 farms were positive (68.3%). The results showed that cattle from Lima dairy farms have moderate individual prevalence and high intra-herd prevalence against N. caninum.
Start page
916
End page
922
Volume
29
Issue
3
Language
Spanish
OCDE Knowledge area
Ciencia veterinaria
Zoología, Ornitología, Entomología, ciencias biológicas del comportamiento
Subjects
Scopus EID
2-s2.0-85054593511
Source
Revista de Investigaciones Veterinarias del Peru
ISSN of the container
16823419
Sponsor(s)
Proyecto «Obtención y caracterización del primer aislado de Neospora caninum cau-sante de abortos y mortalidad neonatal en la producción bovina lechera del Perú, con fines inmunodiagnóstico y vacunal»(Contrato 167-PNICP-PIAP-2015 FINCYT); al Pro-grama Iberoamericano de Ciencia y Tecno-logía para el Desarrollo (CYTED) por el apo-yo financiero brindado a la Red Temática 113RT0469 PROTOZOOVAC: «Diagnósti-co y control de las protozoosis reproductivas del ganado bovino»que permitió la capacita-ción técnica de los investigadorespara la eje-cución del estudio. Del mismo modo, al Dr. Luis Miguel Ortega-Mora y al Grupo SALUVET de la Universidad Complutense deMadrid,España, por ladonaciónde la cepa de Neospora caninum (NcSp7).
Sources of information:
Directorio de Producción Científica
Scopus