Despite its importance in genetic improvement, the fiber density is one of the least known and poorly valued characters, probably due to its difficulty in measuring it. Therefore, a novel procedure has been developed with the objectives to measurament no-invasively, the fiber density (FibDen), fiber bundles by ducts (DucDen) and relationship between N° of fibers/N° of ducts (Fib/Duc) in llamas, alpacas and cattle. The new technique involves four steps: skin and fiber preparation in live animal, capture of skin and fiber amplified images, image storage and image processing and data presentation. A portable digital microscope modified and a computer where two proprietary software developed in C language were installed. The results of the procedure show that the images obtained in alpacas and llamas skin are clear, with up to 7 fibers visible in Huacaya alpacas and Ccara llamas. The averages ± standard error (SE) of FibDen are 23,60±0,36 and 12,73±1,41 fibers/mm 2 , for DucDen are 10,50±0,16 and 6,77±2,26 ducts/mm 2 and for Fib/Duc are 2,41±0,04 and 1,89±0,63 respectively for alpacas and llamas. The results are lowest in cattle and generally each hair emerge of each duct. It is concluded that the procedure allows obtaining at least 3 characteristics of llama and alpaca skin, with the possibility of extending its use in other species.