Conventional methods to diagnose anemia require a blood draw. This generates a great problem in patients due to the fear of contracting a disease through syringes, or sensitivity to this element. The palpebral conjunctiva is an indicator of diseases such as the hordeolum, chalazion, marginal blepharitis, bacterial conjunctivitis, trachoma, and anemia. The palpebral conjunctiva pallor is an indicator of anemia and if we wanted to develop an automatic system, for the non-invasive diagnosis of anemia based on the analysis of photographs of the palpebral conjunctiva, we would need algorithms for the segmentation and analysis of this membrane. In this sense, this research proposes and develops a method for the automatic segmentation of the palpebral conjunctiva using an Android application and image processing techniques. As a result, the success of this segmentation method is 92.2%.