This study aimed to determine the seroprevalence of antibodies against Brucella canis in dogs of the district of Pucusana, Lima, Peru. Blood samples from 202 dogs older than 6 months of age were collected, regardless of breed, sex or reproductive history. The sera was analysed by the technique of agar gel immunodiffusion (AGID). The results showed 21.3 ± 2.9% (43/202) of dogs seropositive for antibodies against B. canis. The variables sex, breed, occurrence of abortion and street presence were evaluated resulting significant association between seropositivity to B. canis and sex (p<0.05).