In this paper, the feasibility of using spectral profiles for modeling water activity aw in white quinoa grains (Chenopodium quinoa Willd.) is studied. For this purpose, five hundred samples of five white varieties were stabilized at different aw values using the isopiestic method. Next, hyperspectral images (HSIs) of ten grains for each combination (variety, aw value), covering the range of 400–1000 nm were acquired, and mean spectral for each grain extracted. Then, due to a linear relationship that the spectral profiles are shown, the modeling was performed with aw values over 0.741 using partial least square regression (PLSR). From total spectra, three hundred spectrum were selected and randomly divided into training and validation sets. The results shown coefficient of determination from 0.59 to 0.834 concluding than for aw over 0.741, HSI + PLSR show potential for aw prediction in white quinoa grains.