Title
Evaluation of reverse transcription-loop-mediated isothermal amplification for rapid detection of SARS-CoV-2
Date Issued
01 December 2021
Access level
open access
Resource Type
journal article
Author(s)
Publisher(s)
Nature Research
Abstract
The main strategy for response and control of COVID-19 demands the use of rapid, accurate diagnostic tests aimed at the first point of health care. During the emergency, an increase in asymptomatic and symptomatic cases results in a great demand for molecular tests, which is promoting the development and application of rapid diagnostic technologies. In this study, we describe the development and evaluation of RT-LAMP to detect SARS-CoV-2 based on three genes (ORF1ab, M and N genes) in monoplex and triplex format. RT-LAMP assays were compared with the gold standard method RT-qPCR. The triplex format (RdRp, M and N genes) allowed obtaining comparable results with de RT-qPCR (RdRp and E genes), presented a sensitivity of 98.9% and a specificity of 97.9%, opening the opportunity to apply this method to detect SARS-CoV-2 at primary health-care centers.
Volume
11
Issue
1
Language
English
OCDE Knowledge area
Biotecnología relacionada con la salud
Bioquímica, Biología molecular
Scopus EID
2-s2.0-85121512181
PubMed ID
Source
Scientific Reports
ISSN of the container
20452322
Sponsor(s)
We thank the members of the National Reference Laboratory of Enteropathogens (INS-Peru) for their technical assistance. This work was supported by the project “Platform for the genotyping of SARS-COV-2 virus”, approved by Institutional Review Boards of Instituto Nacional de Salud of Peru according to D.R. N°191-2020-OGITT/INS.
Sources of information:
Directorio de Producción Científica
Scopus