Title
Isolation and partial characterization of a myotoxin from Bothrops atrox snake venom (Ophidia: Viperidae)
Other title
[Aislamiento y caracterización parcial de una miotoxina del veneno de la serpiente Bothrops atrox (Ophidia: Viperidae)]
Date Issued
01 January 2004
Access level
metadata only access
Resource Type
journal article
Publisher(s)
Facultad de Ciencias Biologicas, Universidad Nacional Mayor de San Marcos
Abstract
A myotoxin from Bothrops atrox snake venom was purified by cationic exchange on CM-Sephadex C-50 with 0,05M ammonium acetate pH 7. The myotoxin is a basic protein and by gel filtration and PAGE-SDS was demonstrated that protein has a molecular weight of 27 kDa and two polipeptides chain of 14 kDa each one. The inoculation of myotoxin in gastrocnemius muscle of white mice produce liberation of creatin kinase as well as myonecrosis. The myotoxin has phospholipasic, anticoagulant and edematic activity, but not hemolytic activity.
Start page
79
End page
86
Volume
11
Issue
1
Language
Spanish
OCDE Knowledge area
ToxicologÃa
Subjects
Scopus EID
2-s2.0-77949901670
Source
Revista Peruana de Biologia
ISSN of the container
15610837
Sources of information:
Directorio de Producción CientÃfica
Scopus