Title
High within-host diversity found from direct genotyping on post-mortem tuberculosis specimens in a high-burden setting
Date Issued
01 October 2021
Access level
open access
Resource Type
journal article
Author(s)
Rodríguez-Grande C.
Rodríguez-Maus S.
Casas I.
Castillo P.
Navarro M.
Rakislova N.
García-Basteiro A.
Carrilho C.
Fernandes F.
Lovane L.
Jordao D.
Ismail M.R.
Lorenzoni C.
Cossa A.
Mandomando I.
Bassat Q.
Menéndez C.
Ordi J.
Muñoz P.
Pérez-Lago L.
García de Viedma D.
Martínez M.J.
Universidad de Barcelona
Publisher(s)
Elsevier B.V.
Abstract
Objectives: To characterize the clonal complexity in Mycobacterium tuberculosis (MTB) infections considering factors that help maximize the detection of coexisting strains/variants. Methods: Genotypic analysis by Mycobacterial Interspersed Repetitive-Unit–Variable-Number Tandem-Repeats (MIRU-VNTR) was performed directly on 70 biopsy specimens from two or more different tissues involving 28 tuberculosis cases diagnosed post-mortem in Mozambique, a country with a high tuberculosis burden. Results: Genotypic data from isolates collected from two or more tissues were obtained for 23 of the 28 cases (82.1%), allowing the analysis of within-patient diversity. MIRU-VNTR analysis revealed clonal diversity in ten cases (35.7%). Five cases showed allelic differences in three or more loci, suggesting mixed infection with two different strains. In half of the cases showing within-host diversity, one of the specimens associated with clonal heterogeneity was brain tissue. Conclusions: Direct MTB genotyping from post-mortem tissue samples revealed a frequent within-host Mycobacterium tuberculosis diversity, including mixed and polyclonal infections. Most of this diversity would have been overlooked if only standard analysis of respiratory specimens had been performed.
Start page
1518.e5
End page
1518.e9
Volume
27
Issue
10
Language
English
OCDE Knowledge area
Otras ciencias médicas
Sistema respiratorio
Enfermedades infecciosas
Biología celular, Microbiología
Subjects
Scopus EID
2-s2.0-85116147770
PubMed ID
Source
Clinical Microbiology and Infection
ISSN of the container
1198743X
Sponsor(s)
The authors declare no conflicts of interest. This study was funded by ERANET-LAC ( TRANS-TB-TRANS AC16/00057 ; ELAC2015/T08-0664 ), ISCIII ( AC16/00057 , FIS15/01554 , 18/00599 ). The CaDMIA research project (validation of the minimally invasive autopsy tool for cause of death investigation in developing countries) was funded by the Bill and Melinda Gates Foundation (global health grant numbers OPP1067522 ; Q. Bassat) ( www.gatesfoundation.org/ ) and by the Spanish Instituto de Salud Carlos III ( FIS, PI12/00757 ; C. Menendez) ( https://portalfis.isciii.es ). Data analysis was supported by the CaDMIA plus research project, funded by the Bill and Melinda Gates Foundation (global health grant numbers OPP1128001; J. Ordi) ( www.gatesfoundation.org/ ) and the Spanish Instituto de Salud Carlos III (Acciones CIBER; C. Menendez) ( www.ciberisciii.es/ ). ISGlobal is a member of the CERCA Programme, Generalitat de Catalunya ( http://cerca.cat/en/suma/ ). No funding bodies had any role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. This study was also partially supported by grant 2017 SGR 794 from the Agència de gestió Agència de Gestió d’Ajuts Universitaris i de Recerca (AGAUR)—Departament d’Empresa i Coneixement, Generalitat de Catalunya . ISGlobal receives support from the Spanish Ministry of Science and Innovation through the ‘Centro de Excelencia Severo Ochoa 2019–2023’ Program ( CEX2018-000806-S ), and support from the Generalitat de Catalunya through the CERCA Program. CISM is supported by the Government of Mozambique and the Spanish Agency for International Development (AECID) .
Sources of information:
Directorio de Producción Científica
Scopus