The extent of the potato gene pool, with its abundant landrace diversity and numerous wild relatives, offers a wide range of options for prospecting, prebreeding, and niche market development. Landraces are still widely produced by smallholder farmers in the center of origin and are a key component of complex production systems that cover multiple agroecologies and production objectives. Sustainable future conservation and use will require attention to systematic monitoring of potato genetic diversity, gap analysis, screening, and prebreeding. Modern and traditional uses are both main drivers behind the rational use of potato biodiversity. Both offer promise for the scientific advancement of potato chemistry and technology.