Four ejaculates were collected at 15 day intervals from six adult alpacas using an artificial vagina inside a dummy. Data were analyzed using factorial design. The mean citric acid concentration was 4.3 mg dl-1 and mean fructose concentration 5.0 mg dl-1, with no differences (P > 0.05) between age and interval of semen collection. Mean ejaculate volume was 1.4 ml, and sperm concentration was 150 354 spermatozoa ml-1, with no differences (P > 0.05) attributed to age and interval of semen collection. Alpaca seminal plasma appears to have low concentrations of citric acid, fructose and semen concentration compared with other livestock species. © 1995.