The purpose of this study is to develop an overview of the Latin American women-owned business environment. Documentary research was conducted based on the factors of the Integrated Framework Assessment for an Enabling Environment for Women-Owned Businesses developed by the International Labor Organization, including access to credit and financial services, institutionalism, associations and women entrepreneurs’ networks, legal and regulatory framework, the distribution of the use of time, citizen security and gender-based violence, and promotion of entrepreneurship. The study concludes that even though there have been important initiatives related to these factors in Latin America and these issues have become important in political agendas, there has been uneven progress in different countries.