Title
Immunodiagnosis of human fascioliasis with Fasciola hepatica cysteine proteinases
Date Issued
1999
Access level
restricted access
Resource Type
journal article
Author(s)
Publisher(s)
Royal Society of Tropical Medicine and Hygiene
Abstract
The immunodiagnosis of human infections with Fasciola hepatica using purified parasite cysteine proteinases as antigens is reported in this paper. IgG enzyme-linked immunosorbent assays (ELISAs) with 2 cysteine proteinases of 26 kDa (Fas1) and 25 kDa (Fas2), obtained from the regurgitated material of adult worms, were evaluated with serum samples from 38 patients infected with F. hepatica, from 54 patients with other parasitic infections and from 46 healthy volunteers. The diagnostic sensitivities for detection of F. hepatica infection at a serum dilution of 1 in 500 were 89% and 95% with Fas1 and Fas2 respectively. The specificity of the ELISAs was 98% for Fas1 and 100% for Fas2. IgG ELISA with Fas2 is a highly sensitive and specific assay for the immunodiagnosis of human fascioliasis.
Start page
54
End page
57
Volume
93
Issue
1
Number
55
Language
English
Scopus EID
2-s2.0-0033007745
PubMed ID
Source
Transactions of the Royal Society of Tropical Medicine and Hygiene
ISSN of the container
0035-9203
Sponsor(s)
Acknowledgements We thank Dr David Dunne from the Depattment of Pathol- ogy of Cambridge University for the gift of serum from patients infected with Schismsomam ansonia nd Dr Elba Miranda for the generous gift of serum from patients with other parasitic infections. Thanks to Drs Humberto Guerra and Ysabel Montova for valuable suggestionst o improve this communication. fiis work was partially supported by a grant no. 0114-02-93-01 from the Peruvian Council for Science and Technology (CONCYTEC).
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