This study was conducted to determine whether the new disinfectant Aviclor (HidroAll do Brazil Ltda., Valinhos, São Paulo, Brazil), a formulation containing sodium dichloroisocyanurate, acetylsalicylic acid, sodium chloride, and a dye pigment, was effective in killing a group of selected pathogens obtained from chickens. The use of Aviclor completely eliminated several viruses, including infectious laryngotracheitis virus, infectious bronchitis virus, infectious bursal disease virus, and rotavirus, at disinfectant concentrations in the range of 0.5 to 0.77 g/L, with exposure times between 5 and 10 min. In addition, Pseudomonas aeruginosa, Escherichia coli, Salmonella enterica serovar Enteritidis phage type 4, Clostridium perfringens, and Staphylococcus aureus were killed after treatments with Aviclor concentrations ranging from 0.67 g/L for 15 min to 0.93 g/L for 20 min. Pseudomonas aeruginosa was the most sensitive to the product, and S. aureus was the most resistant to the disinfectant; the concentration required for eliminating the bacteria was 1.067 g/L for 15 min. We conclude that because of the virucidal and bactericidal activities of Aviclor and the short effective exposure times, together with the low corrosive action and the absence of toxicity to birds and the environment, Aviclor is suitable as a disinfectant for poultry production. © 2010 Poultry Science Association, Inc.