Introduction: Short Tandem Repeat (STR) markers are used in forensic practice due to their great discriminative power and help to identify individuals genetically. At least 20 STRs are currently used in Peru. Objective: The objective of the present study is to determine the allelic frequencies of 21 genetic markers of type STR (D3S1358, vWA, D16S539, CSF1PO, TPOX, D8S1179, D21S11, D18S51, D2S441, D19S433, TH01, FGA, D22S1045, D5S818, D13S317, D7S820, SE33, D10S1248, D1S1656, D12S391 and D2S1338) in a mixed race Peruvian population. Materials and methods: The allelic frequencies were obtained from 300 unrelated individuals from different provinces of Peru. A statistical analysis was performed on the data obtained. Results and conclusions: All loci, except for D10S1248 and D2S1338, were in Hardy-Weinberg equilibrium. The most frequent allele of the D3S1358 marker is 15, with 56.33% in the Peruvian population and with 34.65% in the Hispanic population, while the alleles less frequent in the Peruvian population were 11 and 19, and in the Hispanic one, it was the 9 allele, which the Peruvian population does not have. The vWA marker of the Peruvian population does not have the 11, 12, and 17.3 alleles seen in the Hispanic population. The PD is greater than 0.9999999 and the PE is 0.99999997. When comparing the allelic frequencies of the Peruvian mixed-race population and the Hispanic frequencies of the manual of Globalfiler by Life Technologies For Forensic or Paternity 2013, differences are observed in the allelic frequencies of the markers.