Title
Comparative phenotipic survey of microbial consortia with chitinolytic activity from marine silts and rhizosphere
Other title
[Estudio fenotípico comparativo de consorcios microbianos con actividad quitinolítica de sedimentos marinos y rizósfera]
Date Issued
01 January 2001
Access level
open access
Resource Type
journal article
Author(s)
Publisher(s)
Universidad Nacional Mayor de San Marcos, Fac. Of Biological Sci.
Abstract
Four samples of silts were collected from the bottom and the bank of the beach "Marbella" in Callao and four samples of cultivated land were taken at level of rhizosphere obtained from a vineyard ol "Grocio Prado" Grassland's town in the Country of Chincha. The microbial heterotrophics populations with chitinolytic activity present in these environments was determined quantitatively, being described its main phenotypic characteristics. For the quantification of the populations the method of the Most Probable Number (NMP) was used. The tubes with positive growth were sowed in tubes with Granulated Chitin Agar and Colloidal Chitin Agar that were incubated at environmental temperature for two weeks. With the developed colonies the following tests were carried out: Oxidation/Fermentation of Glucose, Oxidase, Catalase, motility, presence of flagellum, tintoreal capacity, production of spores and semiquantitative chitinolytic activity. The phenograms were elaborated with the NTSYS v. 1,7 program. The biggest density of chitinolytic bacteria was found in rhizosphere reaching levels of >1,6 x 105, as long as in marine silts the density oscillated among 2,2 x 102 to 1,6 x 10J NMP/g. After the analysis of clustering of the phenotypes shown by the studied consortia it was found that they presented distinctive characteristic, exhibiting both of them a great phenotypic diversity.
Start page
70
End page
79
Volume
8
Issue
1
Language
Spanish
OCDE Knowledge area
Biología celular, Microbiología
Biología marina, Biología de agua dulce, Limnología
Subjects
Scopus EID
2-s2.0-84986232798
Source
Revista Peruana de Biologia
ISSN of the container
15610837
Sources of information:
Directorio de Producción Científica
Scopus