Title
Yeast metabolic state identification by fiber optics spectroscopy
Date Issued
21 July 2009
Access level
metadata only access
Resource Type
conference paper
Author(s)
Castro C.C.
Silva J.S.
Martins R.C.
Instituto Nacional de Engenharia Tecnologia e Inovação
Abstract
In this manuscript we explore the feasibility of using LWUV-VIS-SWNIR (340 - 1100 nm) spectroscopy to classify Saccharomyces cerevisiae colony structures in YP agar and YPD agar, under different growth conditions, such as: i) no alcohol; ii) 1 % (v/v) Ethanol; iii) 1 % (v/v) 1-Propanol; iv) 1 % (v/v) 1- butanol; v) 1 % (v/v) Isopropanol; vi) 1 % (v/v) (±)-1-Phenylethanol; vii) 1 % (v/v) Isoamyl alcohol; viii) 1 % (v/v) tert-Amyl alcohol (2-Methyl-2-butanol); and ix) 1 % (v/v) Amyl alcohol. Results show that LWUV-VIS-SWNIR spectroscopy has the potential for yeasts metabolic state identification once the spectral signatures of colonies differs from each others, being possible to acheive 100% of classification in UV-VIS and VIS-SWNIR. The UV-VIS region present high discriminant information (350-450 nm), and different responses to UV excitation v/ere obtained. Therefore, high precision is obtained because UV-VIS and VIS-NIR exhibit different kinds of information. In the future, high precision analytical chemistry techniques such as mass spectroscopy and molecular biology transcriptomic studies should be performed in order to understand the detailed cell metabolism and genomic phenomena that characterize the yeast colony state.
Start page
169
End page
178
Language
English
OCDE Knowledge area
Micología Bioquímica, Biología molecular
Scopus EID
2-s2.0-67650526338
Resource of which it is part
BIOSIGNALS 2009 - Proceedings of the 2nd International Conference on Bio-Inspired Systems and Signal Processing
ISBN of the container
978-989811165-4
Conference
2nd International Conference on Bio-Inspired Systems and Signal Processing, BIOSIGNALS 2009
Sources of information: Directorio de Producción Científica Scopus