Title
Screening of expression libraries using ELISA: Identification of immunogenic proteins from Tityus bahiensis and Tityus serrulatus venom
Date Issued
01 January 2001
Access level
metadata only access
Resource Type
journal article
Author(s)
Kalapothakis E.
Jardim S.
Magalhães A.C.
Mendes T.M.
De Marco L.
Afonso L.C.C.
Fundação Ezequiel Dias
Abstract
The present report describes the use of ELISA with cDNA expression libraries in the identification of immunogenic proteins. The methodology described was applied using libraries constructed with mRNA isolated from Tityus serrulatus and Tityus bahiensis venom glands. In addition we describe for the first time the sequence of a neurotoxin from Tityus bahiensis venom gland named TbTx5 whose amino acid sequencing showed 93% similarity with the Tityus bahiensis TbTx IV-5 neurotoxin. The methodology described can be used for the generation of an immunogenic bank in order to contribute to genome and proteome projects. Copyright (C) 2000 Elsevier Science Ltd.
Start page
679
End page
685
Volume
39
Issue
5
Language
English
OCDE Knowledge area
Toxicología
Subjects
Scopus EID
2-s2.0-0035238880
PubMed ID
Source
Toxicon
Resource of which it is part
Toxicon
ISSN of the container
00410101
Sponsor(s)
Funding text
The work was supported by PADCT, FAPEMIG, CNPq and PRONEX.
Sources of information:
Directorio de Producción Científica
Scopus