Urbanization usually reduces bat richness; however, the presence of green areas within cities and peripheral rural areas in arid ecosystems may provide microhabitats for some species. Light pollution is a major feature of urbanization, but its impact on bat behavior appears to be species-specific and previous studies have documented contrasting responses. Moreover, the effect of urbanization on bat species has been poorly studied in arid regions. We assessed the effect of artificial night light intensity (as a proxy of urbanization) on both bat occupancy and the acoustic space used (ASU) in an urbanization gradient in Peruvian central coast, based on passive acoustic recorders. We collected 26,169 recordings from 19 sites which resulted in 579 independent detections of 15 bat species. Variation in both ASU and species richness was best explained by artificial night light intensity. Species-specific effects of the artificial night light intensity based on a multi-species occupancy modeling showed that this covariate had a negative effect on occupancy for most of the bat species (12 species). ASU and both observed and posterior bat species richness were positively correlated, suggesting that ASU can be used as a proxy of bat richness. This study provides evidence that both bat richness and occupancy decrease with artificial light intensity; nevertheless, eight species used urban areas, similar to results found in other cities around the world.