Title
Use of a reliable PCR assay for the detection of Neisseria gonorrhoeae in Peruvian patients
Date Issued
01 January 2006
Access level
open access
Resource Type
journal article
Author(s)
Publisher(s)
Blackwell Publishing Ltd
Abstract
Neisseria gonorrhoeae is the most common sexually transmitted disease-causing bacterium worldwide. An in-house PCR assay targeting the carbamoyl-phosphate synthase subunit A (carA) gene was developed for the specific detection of N. gonorrhoeae in clinical specimens. Samples from 605 patients were cultured on selective medium and assayed by PCR in a double-blind fashion. Of 605 urethral/cervical samples analysed, 13 were PCR-positive, of which 11 were culture-positive. The PCR showed a sensitivity and specificity of 100% and 99.7% with these samples. PCR targeting the carA gene appears to be a reliable method for the detection of N. gonorrhoeae in clinical specimens. © 2006 European Society of Clinical Microbiology and Infectious Diseases.
Start page
809
End page
812
Volume
12
Issue
8
Language
English
OCDE Knowledge area
Dermatología, Enfermedades venéreas
Enfermedades infecciosas
Subjects
Scopus EID
2-s2.0-33745590844
PubMed ID
Source
Clinical Microbiology and Infection
ISSN of the container
1198743X
Sponsor(s)
JT was supported by the Fogarty International Center award T35AI107646. The authors are grateful to V. Chavez, Y. Salas, C. Quiroz and A. Culotta for their contributions to the development of the present study, and J. L. Portilla of the Laboratorio de Bacterias de Transmisió Sexual, Centro Nacional de Salud Pública, Instituto Nacional de Salud, Lima, Peru for the Neisseria strains. We appreciate the technical assistance of J. P. Castillo, M.-C. Camila, J. B. Phu and D. Sara.
Sources of information:
Directorio de Producción Científica
Scopus