Microbial fuel cells are providing great opportunities for electricity generation through the use of organic waste. This research work gives an environmentalist solution by using molasses waste for the generation of bioelectricity through single-chamber microbial fuel cells using zinc and copper electrodes; manufactured inexpensively. The maximum voltage generated was 0.953 ± 0.142 mV and the current was 1.73 ± 0.13 mA, all cells showed a slightly acidic pH during the 30 days of monitoring. The conductivity values increased until day 22 (111.156 ± 8.45 mS/cm) and the Brix degrees values decreased from 20.07 ± 0.57 ° Brix to 11.33 ± 2.18 ° Brix. The maximum power density found was 5.45 ± 0.31 W/cm2 with a current density of 308.06 mA/cm2