The study aimed to evaluate the effects of the inclusion, alone or in combination, of phytase and 25-hydroxycholecalciferol vitamin (25-OHD3) on the productive performance and tibia characteristics of male broilers from 1 to 21 day of age. A total of 200 male chicks of the Cob 500 Line were distributed in four treatments with five replicates and 10 birds per repetition. The treatments were: T1, Control; T2, Isonutritive as T1 considering phytase matrix (0.178% Ca and 0.15% AP available phosphorous); T3, Isonutritive as T1 considering 25-OHD3 matrix (0.1% Ca and 0.05% AP) and T4, Isonutritive as T1 considering both phytase and 25-OHD3 matrixes. Broilers were weighed and feed intake recorded on days 7, 14, and 21. On day 21, lengths and weights of tibias of twelve chicks per treatment were measured. Left tibias were tested for ash, calcium and phosphorus contents. No statistical difference was found in body weight, daily weight gain or carcass yield between treatments. T3 showed lower bone density, shorter and lighter tibias than those of other treatments (p<0.05). Ash contents and calcium concentrations in tibias from birds fed T2 or T4 were lower than those on T1 or T3 (p<0.05). Phosphorus concentration in tibia from broilers fed T2, T3, and T4 were similar to those fed T1. In conclusion, the phytase enzyme, vitamin 25- OHD3 and the combination of both as a supplement in diets of broilers neither had positive effect on the productive performance of broilers nor contributed to effective mineralization of the tibia.