The aim of this study was to determine the prevalence and parasite load of Fasciola hepatica in llamas and alpacas of 12-48 months of age in the districts of Masma Chicche and Llocllapampa, Jauja, Peru, and to determine the association between the prevalence of F. hepatica with sex and age. Two hundred fecal samples were collected from llamas (n= 97) and alpacas (n= 103) in October 2011. The samples were analyzed by the spontaneous sedimentation technique and the parasite load by the modified McMaster method. The prevalence of F. hepatica in llamas was 49.5% and in alpacas was 73.8% without statistical differences due to sex and age. The parasite load was 12.6 and 19.9 eggs per gram of faeces (epg) in llamas and alpacas respectively.