Title
Development of an antibody-detection ELISA for Fasciola hepatica and its evaluation against a commercially available test
Date Issued
01 April 2005
Access level
metadata only access
Resource Type
journal article
Author(s)
Publisher(s)
Elsevier
Abstract
An ELISA was developed for the detection of Fasciola hepatica antibody in serum of cattle. The assay was applied to sera from 258 naturally infected cattle, 256 non-infected cattle and six calves experimentally infected with F. hepatica. The diagnostic sensitivity and specificity of the ELISA test was 98% (95% confidence intervals, 96-100%) and 96% (95% confidence intervals, 93-98%) respectively at a cut-off value of 15% positivity. The results using sera from the experimentally infected calves showed that antibodies were first detected 2-4 weeks after infection. The ELISA test was also compared to the commercially available Bio-X bovine F. hepatica ELISA kit. A subset of 39 positive sera and 47 negative sera were selected from the samples used to evaluate the in-house test. The results indicated that the agreement between the two tests was almost perfect (k statistic = 0.82). © 2004 Elsevier Ltd. All rights reserved.
Start page
177
End page
181
Volume
78
Issue
2
Language
English
OCDE Knowledge area
Ciencia veterinaria
Ciencia animal, Ciencia de productos lácteos
Parasitología
Subjects
Scopus EID
2-s2.0-10244254018
PubMed ID
Source
Research in Veterinary Science
ISSN of the container
00345288
Sponsor(s)
This investigation was carried out as part of a PhD project that was funded by the Ministry of Science, Research and Technology of Iran. The authors are grateful to the staff of the Faculty of Veterinary Science of the National University of Cajamarca for collecting the samples and to the farmers of Cajamarca, Peru, for their help. We are also grateful to the Cheshire farmers for their collaboration and to Dr John Claxton for reviewing the manuscript.
Sources of information:
Directorio de Producción Científica
Scopus