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Browsing Publications by Department "34196 Montpellier Cedex 5"
  • Publication
    High dietary arachidonic acid levels affect the process of eye migration and head shape in pseudoalbino Senegalese sole Solea senegalensis early juveniles
    ( 2013-11-01)
    Boglino A.
    ;
    Wishkerman A.
    ;
    DARIAS CACERES, MARIA
    ;
    Andree K.
    ;
    de la Iglesia P.
    ;
    Estévez A.
    ;
    Gisbert E.
    The effect of high dietary levels of arachidonic acid (ARA) on the eye migration and cranial bone remodelling processes in Senegalese sole Solea senegalensis early juveniles (age: 50days post hatch) was evaluated by means of geometric morphometric analysis and alizarin red staining of cranial skeletal elements. The incidence of normally pigmented fish fed the control diet was 99·1±0·3% (mean±s.e.), whereas it was only 18·7±7·5% for those fed high levels of ARA (ARA-H). The frequency of cranial deformities was significantly higher in fish fed ARA-H (95·1±1·5%) than in those fed the control diet (1·9±1·9%). Cranial deformities were significantly and negatively correlated with the incidence of normally pigmented animals (r2 = -0·88, P < 0·001, n = 16). Thus, fish displaying pigmentary disorders differed in the position of their eyes with regard to the vertebral column and mouth axes, and by the interocular distance and head height, which were shorter than in fish not displaying pigmentary disorders. In addition to changes in the positioning of both eyes, pseudoalbino fish showed some ARA-induced osteological differences for some of the skeletal elements from the splanchnocranium (e.g. right premaxillary, dentary, angular, lacrimal, ceratohyal and branchiostegal rays) and neurocranium (e.g. sphenotic, left lateral ethmoid and left frontal) by comparison to normally pigmented specimens. Pseudoalbino fish also had teeth in both lower and upper jaws. This is the first study in Pleuronectiformes that describes impaired metamorphic relocation of the ocular side eye, the right eye in the case of S. senegalensis, whereas the left eye migrated into the ocular side almost normally. © 2013 The Fisheries Society of the British Isles.
  • Publication
    Isolipidic diets differing in their essential fatty acid profiles affect the deposition of unsaturated neutral lipids in the intestine, liver and vascular system of Senegalese sole larvae and early juveniles
    (Elsevier Inc., 2012-05-01)
    Boglino A.
    ;
    Gisbert E.
    ;
    DARIAS CACERES, MARIA
    ;
    Estévez A.
    ;
    Andree K.B.
    ;
    Sarasquete C.
    ;
    Ortiz-Delgado J.B.
    How lipid content and composition in the diet is utilized by the various organs and tissues of fish is reflected in their structure, such as the intestine through which dietary lipids are digested and absorbed, the vascular system which is involved in their transport, and the liver where lipids are stored and metabolized. However, no study has been conducted to compare the effect of different diets containing different levels of highly unsaturated fatty acids (HUFA) and essential fatty acids (EFA) on lipid deposition in fish larvae and early juveniles. Thus, we evaluated the effects of six isolipidic diets (enriched Artemia salina), differing in their fatty acid profile, on the lipid accumulation patterns in selected target tissues (intestine, liver and vascular system) in Senegalese sole (Solea senegalensis) larvae and early juveniles. Results showed that the profile of fat accumulation in these three tissues was significantly affected by the dietary treatments, the developmental stage of the fish (premetamorphosis, metamorphosis or postmetamorphosis), as well as by the interaction between these two factors that were responsible for changes in the histological organization of the tissues. Histological results revealed that a slight variation in the EFA levels (e.g. EPA, DHA or ARA) or in their ratios (EPA/DHA, ARA/EPA, ARA/DHA, (n-3)/(n-6) HUFA, OA/PUFA) modified the metabolism of lipids and disrupted the pattern of lipid accumulation in the target tissues, leading to intestinal and hepatic steatosis. © 2012 Elsevier Inc.